mRNA-In™ mRNA Transfection Reagent - Various Sizes

NEW!- A Universal Transfection Reagent for High Efficiency mRNA Delivery mRNA-In Transfection Reagent was developed by members of the scientific team that invented Lipofectamine and Lipofectamine2000 andspecifically formulatedfor in vitro mRNA delivery.Maximum RNA delivery isachievedwith mRNA-In using less amount mRNA than other reagents, reducing experimental costs and significantly lowering toxicity. Across a wide range of cell types, mRNA-In has shown to produceexceptionally high transfection efficiency while maintaining optimal cell health and viability. Features: Requires low amounts of RNA- reduce cost and potential off-target toxicity effects with low amounts of RNA requirements. High expression, low toxicity- achieve high mRNA efficiency while maintaining optimal cell health for uncompromised experimentresults. Robust, versatile performance - produce efficienttransfection across awide range of cell type, including stem cells and primary cells. ​ Easy-to-follow "Quick" protocol Application: mRNA-In Transfection Reagent is ideal for mRNA delivery forvarious applications, such as protein expression andiPSC-reprogramming. Data below also shows mRNA-In significantly outperforms competitor reagents, typically achieving 90% or greatertransfection efficiency across a wide range of cell types. mRNA-In DeliversMaximum Transfection Efficiency using Low Amounts of mRNA Across a Range of Cell Types Databelowshow commonly usedcell typestransfected with mRNA-In Transfection Reagent using various amounts of mRNA. Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNAcontaining 5-methlycytosine and psuedouridine were complexed with various amountsof mRNA-Inina total of 50l of OptiMEM. For each experimentmRNA /mRNA-In complexeswereadded to cells, mixed, and incubated at 37C in 5% CO2. Cells wereobserved24 hours following transfection. SH-SY5Y Neuroblastoma Cells were transfected with100ng GFP-mRNA/1L mRNA-In Transfection Reagent Human Adult Fibroblasts were transfected with 200ng GFP-mRNA/1L mRNA-In Transfection Reagent HUVECcells were transfected with100ng GFP-mRNA/1L mRNA-In Reagent Transfection Reagent iPSC-Derived Neural Stem Cells were transfected with 50ng GFP-mRNA /0.25L mRNA-In Transfection Reagent NCRM-1 iPSCwere transfected with 100ng GFP-mRNA/1L mRNA-In Transfection Reagent mRNA-In DeliversHigher GFP Expression and Outperforms theLeadingCompetitorTransfection Reagent Below, SH-Sy5Y (neuroblastoma cells), HDF (human dermal fibroblasts), HUVEC (human umbilical vein endothelial cells), HeLaand adiposed-derived Mesenchymal Stem Cells (MSCs)were transfected with mRNA-In or MessengerMAX (Life Technologies)using low amounts ofmRNA (100ng).Cells were plated in 24-well plates to give 60-70% confluence the day of transfection. GFP-mRNAcontaining 5-methlycytosine and psuedouridine were complexed with the various amountsof mRNA-In (see chart legends). RNA/reagent complexes wereadded to the cells, mixed, and incubated at 37C in 5% CO2 overnight. Cells wereanalyzedby a fluorescence plate reader and microscopy 24 hours post-transfection. Error bars represent the standard deviation of triplicate wells.The data below shows mRNA-Inprovidessignificantlyhigher transfection efficiency than the competitor reagent.