iQuant™ High Sensitivity RNA Quantitation Kit 5-100 ng 1,000 assays

The next generation of nucleic acid quantitation DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Generation Sequencing, PCR, cloning, and DNA transfections. GeneCopoeia has developed iQuant DNA or RNA quantitation kits, which allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts. FEATURES Ultra-sensitive 100 times more sensitive than NanoDrop. Broad dynamic range Sensitivie over four orders of magnitude. More accuracy Unaffected by proteins and free nucleotides. Easy to use Mix-and-read format. Instrument compatibility Assay can be performed using a fluorescence plate reader or a fluorometer such as Qubit. Simple Assay Workflow The kits are designed as a Mix-and-read format. Simply mix the iQuant reagent with standards or user samples, vortex assay tubes for 2~3 seconds, and incubate at room temperature for 2 min, the samples are ready to be quantified using Qubit fluorometer. iQuant NGS dsDNAAssays The iQuantNGS dsDNAassays are designed specifically for an easy and accurate quantitation of dsDNA. The assays are highly selective for dsDNA over RNA, and offer advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. GeneCopoeia offers two iQuantNGS dsDNAassays: iQuantNGS-HS dsDNAassay and iQuantNGS-BR dsDNAassay. The iQuantNGS-HS dsDNAassay can be used to measure dsDNA concentration between 10 pg/mL and 100 ng/mL, and the iQuantNGS-BR dsDNAassay can be used to measure dsDNA concentration between 100 pg/mL and 1,000 ng/mL. The assays are ideal for DNA quantitation in NGS library. Qubit dsDNA HS iQuant NGS-HS Qubit dsDNA BR iQuant NGS-BR Sample #1 12.5 12.6 11.9 11.8 10.7 10.2 10.2 10.5 Sample #2 12.7 12.7 11.5 11.9 11.3 11.1 9.68 9.52 Sample #3 29.2 28.4 24.5 24.8 25.2 24.6 23.6 23.2 Sample #4 37.0 36.6 30.8 31.2 30.6 29.8 32.2 32.0 Sample #5 51.8 51.8 43.4 44.2 45.6 44.2 47.0 47.2 Sample #6 17.7 17.6 15.9 15.7 14.6 14.3 16.1 15.8 Download Product Sheet:https://www.vitascientific.com/image/data/ABMN1020-1-dsDNA Assays.pdf iQuantTM RNA Quantitation Kits The iQuantTM RNA Quantitation Kits are highly selective for RNA in the present of DNA, and offer advantages instability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The assays are well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides.