cdc25A (Phospho-Ser76) Rabbit Polyclonal Antibody, 100 ug

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. Product datasheet: Overview Product Description Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. Tested Applications WB IHC Image Western blot analysis of extracts from Hela cells untreated or treated with TNF- and CA using cdc25A (Phospho-Ser76) Antibody #11138. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76) Antibody #11138 (left) or the same antibody preincubated with blocking peptide (right). Species Reactivities Human Mouse Rat Immunogen Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A. Properties Storage Instructions Store at -20C Clonality Polyclonal Antibody References: CDC25A-inhibitory RE derivatives bind to pocket adjacent to the catalytic site. Tsuchiya A, et al. Mol Biosyst, 2013 May. PMID 23467652 Cdc25A promotes cell survival by stimulating NF-B activity through IB- phosphorylation and destabilization. Hong HY, et al. Biochem Biophys Res Commun, 2012 Apr 6. PMID 22417828 CDC 25A gene 263C/T, -350C/T, and -51C/G polymorphisms in breast carcinoma. Karagoz ID, et al. Tumour Biol, 2010 Dec. PMID 20614206 CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples. Yamashita Y, et al. J Neurooncol, 2010 Oct. PMID 20217459 Hypoxia-mediated regulation of Cdc25A phosphatase by p21 and miR-21. de Oliveira PE, et al. Cell Cycle, 2009 Oct 1. PMID 19738433 Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins. Galaktionov K.I., Beach D. Cell 67:1181-1194(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-182. Varmeh-Ziaie S., Manfredi J.J. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). NIEHS SNPs program Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PHE-88. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph. Alternative splicing in the regulatory region of the human phosphatases CDC25A and CDC25C. Wegener S., Hampe W., Herrmann D., Schaller H.C. Eur. J. Cell Biol. 79:810-815(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-234 (ISOFORM 2).