X-CLARITY™ ETC Control Tower (ETCII) (C30002) Cell Biology Logos Biosystems

Description

Technology

CLARITY: Clear Lipid-exchanged Anatomically Rigid Imaging/immunostaining-compatible Tissue Hydrogel

X-CLARITY, an improved Electrophoretic tissue clearing procedure that can speed up the tissue process considerably.

“See-Through Brain” — as the originally published article in the Journal Nature in May 2013: The CLARITY method was developed at Stanford University. This cutting-edge tissue processing technology has the potential to revolutionize 3D tissue imaging and more by MAKING OPAQUE TISSUE TRANSPARENT! Originally developed for 3-D imaging in brain, the CLARITY method also may be applied to other solid organs for use in 3-D imaging applications.

Here is a video demonstration of improved CLARITY procedure:

First published by researchers at Stanford University in 2013 (Chung et al, Nature 497:332-337), the CLARITY method infuses the brain with a hydrogel monomer. The polymerized hydrogel provides an infrastructure to support the form and structure of the brain. The whole brain or other organ is then clarified via electrophoresis, removing the cellular lipids. This innovative process yields a transparent tissue and allows unparalleled 3D imaging of complex cellular networks and provides an alternative to sectioning for high-res tissue imaging.

CLARITY Procedure Diagram

X-CLARITY Electric Tissue Clearing (ETC) System

The X-CLARITY™ is the first commercial turn-key system that is optimized to perform the groundbreaking CLARITY method and will greatly simplify the CLARITY tissue clearing for your lab. Many researchers have been unsuccessful in replicating the CLARITY method due to its complex, do-it-yourself procedure. The X-CLARITY ETC eliminates the need to build your own device and has a simple to perform work flow and high rate of success for end users. X-CLARITY incorporated many improvements to the original ETC process.

Key system features:

Timed polarity switching electrophoresis (Patent pending);

Fully integrated temperature control with built-in temperature probe and buffer re-circulating pump;

ETC chamber with optimized buffer flow and plate electrodes;

System Components:

– X-CLARITY ETC Chamber (1 ea)

– X-CLARITY ETC Controller (1 ea)

– X-CLARITY Pump (1 ea)

– X-CLARITY Reservoir (1 ea)

– Tssue Container (5 ea)

– Tissue Container Holder (1 ea)

– Electrophoretic Tissue Clearing Solution (12 X 1L)

Based on the original CLARITY technique and licensed from Stanford University, the X-CLARITY ETC System delivers a completely optimized process. Instead of spending weeks to complete the procedure, the CLARITY ETC XL enables researchers to finish the process in days with high degree of success. Logos BioSystem developed a patent pending technology that includes polarity switching electrophoresis, optimized buffer exchange and a temperature controlled ETC chamber. Many beta-testers have achieved successful tissue clearing on their 1st try!

X-CLARITY Product Brochure

Contact us for more information.

Instruction videos

X-CLARITY system introduction Set up guide: system components Set up guide: cable connections

Set up Guide: connecting ETC chamber Set up guide: Pump operation Set up guide: ETC chamber set up

Set up guide:ETC chamber set up 2 Set up guide: ETC control unit

Accessories and Related Products

Catalog No. Description Unit

Consumables C13001 Electrophoretic Tissue Clearing Solution (1L per bottle) 12 X 1L

C13101 X-CLARITY Mounting Solution, 25ml 1 ea

C13102 X-CLARITY Mounting Solution, Value Pack 10 X 25 ml

C13107 X-CLARITY Mounting Solution, Bulk Pack 20 X 25 ml

Accessories C12001 Tissue Container Holder (For mouse whole brain), 20 units 1 ea

C12002 Container Holder for 1 Tissue Container 1 ea

C12004 Mouse Brain Slice Holder (Chamber Height: 4 mm) 1 ea

C12007 Whole Rat Brain Holder 1 ea

Spare parts C10101 X-CLARITY ETC Chamber 1 ea

C30002 X-CLARITY ETC Control Tower (ETCII) 1 ea

C30004 X-CLARITY Buffer Reservoir for ETCII 1 ea

C32001 X-CLARITY Tray for ETCII 1 ea

C12101 Multicable (For power/controller) 1 ea

C15001 Replacement Tubes for X-CLARITY™ Tissue Clearing System

(Snap-Lock Connector Tube+Peristaltic Pump Tube) 1 set

C35001 Replacement Tubes for X-CLARITY™ Tissue Clearing System II

(Snap-Lock Connector Tube/Silicone Tube/Peristaltic Pump Tube) 1 set

Useful Links

CLARITY Technology Wikipedia

Original Research Paper Published in the Journal Nature

See-Through Brain: CLARITY technique youtube video

Original Nature Journal News

CLARITY Resource Center