ViroVision™ Rev-A3R5-GFP/Luc HIV Reporter Cells, 1 vial of 5 x 10⁶ cells

HIV Rev-dependent Reporter Cells A new generation of HIV indictor cells The HIV Rev-dependent reporter cellsrepresent a major advancement in the development of HIV indicator cells (Wu et al., 2007). This new reporter system differs dramatically from the common LTR-based reporter cells, which rely solely on the HIV promoter, the long terminal repeat (LTR), to drive reporter expression. While responsive to an early HIV protein, Tat, the LTR is also responsive to cell culture conditions and stimulation by a variety of known and unknown factors, including cytokines, mitogens, HDAC inhibitors, lipopolysaccharide, certain anti-tumor drugs, and free viral proteins (Siekevitz et al., 1987;Sweet et al., 1995). Such non-HIV-dependent reporter expression frequently diminishes reporter specificity and sensitivity. In contrast to the LTR-based reporter cells, our Rev-dependent reporter cells use both LTR and the Rev/RRE interaction to regulate reporter gene expression. This strict requirement for Rev, a viral protein present only in infected cells, drastically improves the reporter specificity and sensitivity. As a result, our Rev-dependent reporter cells are suitable for a broad range of applications, including screening broadly neutralizing antibodies and anti-HIV drugs, and studying HIV cell-cell transmission and host restriction and dependency factors. Derived from CD4 T cells, our reporter cells express native levels of HIV receptors and are natural HIV targets with broad susceptibility to X4, R5, primary HIV isolates, and certain SIV strains. With GFP, Luc, or GFP/Luc detection options, our Rev-dependent cells provide a versatile and flexible platform for HIV research. Highlights Unparalleled sensitivity & specificity:Rev-regulated reporter expression Versatile:GFP & Luciferase dual reporter system Natural HIV target:Derived from human CD4 T cells Physiologically relevant:Natural levels of HIV receptors/co-receptors Broad susceptibility:Susceptible to X4, R5, primary HIV isolates, some SIV Applications Our Rev-dependent reporter cells allow you to easily perform: TCID50 assays: Routine HIV infectivity & quantification Anti-HIV drug screenings via one-step infection Routine EC50/LD50 quantifications of anti-HIV compounds Screenings for broadly neutralizing antibodies (bnAB) (from laboratory and clinical research samples) Neutralizing antibody quantifications HIV cell-cell transmission and HIV drug-resistance studies HIV host restriction factor (HRF) studies HIV host dependency factor (HDF) studies Low-level HIV gene expression assessments HIV pre-integration transcription studies HIV latency and reactivation studies HIV outgrowth detection following reactivation HIV tropism determinations https://virongy.com/products/hiv-rev-dependent-reporter-cells/