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Uracil-DNA Glycosylase (UNG), Cod, 0.1KU, (A611-1B1634-209)

Uracil-DNA Glycosylase (UNG), Cod, 0.1KU, (A611-1B1634-209)

Store / Applications / Bioreagents / Buffers and Chemicals / Chemicals
$158.60
In stock
Product Details

(Amresco 1B1634-0.1KU ) Uracil-DNA Glycosylase (UNG), Cod Complete, irreversible heat-inactivation Prevents carryover contamination in PCR, qPCR, RT-PCR, and qRT-PCR Works with all commercially available master mixes Only a 5 minute incubation step is required prior to amplification In contrast to competing Uracil-DNA Glycosylases, VWR Life Sciences arctic-derived UNG has the distinct advantage of having the low inactivation temperature of 45C, which enables carryover decontamination of one-step reverse transcription reactions with UNG prior to the cDNA synthesis step. Inactivation is irreversible after heating just 20 minutes at 55C or 1 second at 95C, allowing for stability of the cDNA that has incorporated dUTP. The irreversible inactivation feature of VWR Life Science UNG also facilitates greater downstream manipulation and stability of newly synthesized dUTP-containing DNA by PCR, allowing for long-term storage, restriction digestion, cloning, sequencing and hybridization applications. All amplification reactions must use dUTP-containing dNTP mixtures in order for the UNG decontamination method to be effective. Storage/Stability Product is stable at least 2 years when stored frozen (-20 0C). Product may be stored cold (4 8C) for up to 6 months. coli (ung-) by a modified ung gene derived from Atlantic Cod. It degrades uracil-containing single-stranded and double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolyzing the N-glycosidic bond between deoxyribose sugar and the base in uracil. This generates alkaline sensitive apyramidinic sites in the DNA that will be cleaved upon a combination of alkaline conditions and high temperature. Pretreatment of samples with UNG prevents PCR carryover contamination in labs that substitute dUTP in place of dTTP during all amplification reactions. PCR products containing uracil become substrates for UNG and will be degraded if they are present in subsequent reaction mixtures subjected to UNG treatment. Only DNA templates containing thymidine are not degraded by the treatment and will be amplified.

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Uracil-DNA Glycosylase (UNG), Cod, 0.1KU, (A611-1B1634-209)
Store / Applications / Bioreagents / Buffers and Chemicals / Chemicals
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