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TurboTEV Protease, large, 10 mg (100,000 Units) (T0102L)

TurboTEV Protease, large, 10 mg (100,000 Units) (T0102L)

Store / Applications / Protein Expression and Analysis / Protein Isolation, Stabilization and Purification
$4.16
In stock
Product Details

Accelagen's TEV protease, TurboTEV TM , contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly/Ser. TurboTEV Protease is a restriction grade protease that has a robust activity at 4 o C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin. Expression Species and Protein Type: E. coli,Protease Specific Activity:> 10 Units/g. 1 Unit of TurboTEV Protease cleaves >85% of 3 g of control substrate in 1 hour at 30oC. Storage/Handling: Store at -20 o C. Shipped in gel packs. Formulation: 2 mg/ml in 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol Protease Activity: A 49 kDa GST-fusion protein (C) at 1 mg/ml is incubated with TurboTEV or TEV Protease at a ratio of (1) 1:50, (2) 1:100, (3) 1:200 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 27 kDa and 22 kDa. 'TEV' is a competitors TEV Protease product.

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TurboTEV Protease, large, 10 mg (100,000 Units) (T0102L)
Store / Applications / Protein Expression and Analysis / Protein Isolation, Stabilization and Purification
Display prices in: USD
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