TurboNuclease TM is an ultra-pure recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Benzonase) produced in E. coli using a proprietary process. This non-specific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation. Expression Species and Protein Type: E coli,Endonuclease Unit Definition:One unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37oC in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 g of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase Storage: Store TurboNuclease at -20 o C. Endotoxin Level:< 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay. Formulation: 250 units/l in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol TurboNuclease Activity and Purity: 50 g of salmon sperm DNA was incubated with the indicated units of TurboNuclease and another brand of nuclease at 37oC for 30 minutes in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was monitored by agarose gel. TurboNuclease is purified through a proprietary process that achieves purity of >99%. TurboNuclease has no detectable protease activity.
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