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Turbo3C™Protease (HRV 3C Protease), Bulk Size, >100 mg (H0101M)

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Product Details

Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe- Gln- Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant Turbo3C Protease is a restriction grade HRV3C protease that has robust activity at 4 o C and high specific activity. Turbo3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni chelating or Glutathione (GSH) resin. Source Species and Protein Type: E. coli,Protease Specific Activity:> 1 Units/g. 1 Units of Turbo3C (HRV3C) Protease cleaves >95% of 100 g of target protein at 4oC for 16 hours. Storage/Handling: Store at -20 o C. Shipped in gel packs. Formulation: 2 mg/ml in 50 mM Tris-HCl, pH8.0, 150 mM NaCl, 1 mM TCEP, 50% glycerol Protease Activity: A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with Turbo3C (HRV3C) Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4 o C for 16 hours. The cleaved products are 42 kDa and 26 kDa

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Turbo3C™Protease (HRV 3C Protease), Bulk Size, >100 mg (H0101M)
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