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TFB Electro 10B™ High Efficiency Competent Cells in Cuvettes (10 x 0.02 ml) (73602)

TFB Electro 10B™ High Efficiency Competent Cells in Cuvettes (10 x 0.02 ml) (73602)

Store / Applications / Molecular Biology
$123.75
In stock
Product Details

TFB Electro 10B in Cuvettes are pre-dispensed in sterile, 1 mm gap electroporation cuvettes for simple, convenient electro-transformation. The high transformation efficiency of these cells makes them ideally suited for demanding applications such as cDNA or genomic library construction (1). TFB Electro 10B cells are resistant to bacteriophage T1, an especially virulent virus which attacks E. coli, and can be rapidly spread via aerosols. Handling and Storage Upon receipt, store cells immediately at -70Cor below. Competent cells should be thawed on wet ice (~ 10 minutes) and should be used promptly after thawing. Thawed cells can be refrozen by placing them in dry ice although a drop in transformation efficiency of as much as 50% may be observed. Cells stored properly will remain stable for up to 2 years. Genotype F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80/acZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK rpsL nupG - fhuA (T1R). Note: The Δ(mrr-hsdRMS-mcrBC) deletion, together with the mcrA mutation, allow cloning of methylated DNA such as genomic DNA from eukaryotic sources. Quality Control Each lot of TFB Electro 10B cells is tested to verify transformation efficiencies of 1 x 10 10 transformants/g with limiting amounts of supercoiled pUC19 DNA (10 pg). Saturating amounts of DNA (250ng) give 1 x 108 colonies/ 20l reaction. Untransformed cells are also tested for resistance to bacteriophage T5, a standard test for resistance to phage T1, as well as other relevant genetic markers and sensitivities to antibiotics.

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TFB Electro 10B™ High Efficiency Competent Cells in Cuvettes (10 x 0.02 ml) (73602)
Store / Applications / Molecular Biology
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