TFB5competent E. coli cells that are intended to be transformed by heat-shock.The high transformation efficiency and 96-well formatting of these cells make them ideally suited for high-throughput cloning applications (1). TFB5 cells are resistant to bacteriophage T1, an especially virulent virus which attacks E. coli, and can be rapidly spread via aerosols. Handling and Storage Upon receipt, store cells immediately at -70or below. Competent cells in 96-well plates should be thawed in a pre-chilled (0C) metal heating block or on wet ice (~ 5 minutes) and used promptly after thawing. Cells stored properly will remain stable for up to 2 years. Kit Contents TFB5 Competent Cells pUC19 Control DNA (10 pg / l) Genotype F-Φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(r-k, m+k) phoA supE44 - thi-1 gyrA96 relA1 fhuA (T1R). Quality Control Each lot of TFB5 cells is tested to verify transformation efficiencies of 1 x 10 7 transformants per g with limiting amounts of supercoiled pUC19 DNA (25 pg). Untransformed cells are also tested for resistance to bacteriophage T5, a standard test for resistance to phage T1, as well as other relevant genetic markers and sensitivities to antibiotics.
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At IntelixBio, our dedication to advancing healthcare is exemplified through our unparalleled range of medical lab equipment. Each product is meticulously crafted to meet the exacting standards of modern laboratory environments, facilitating seamless integration and operation. Our commitment to quality permeates every aspect of our offerings, ensuring that laboratories can consistently rely on our medical lab equipment to deliver precise and dependable results. With a comprehensive selection that spans from basic laboratory essentials to sophisticated diagnostic instruments, IntelixBio remains the foremost choice for institutions seeking excellence in medical equipment solutions. Trust IntelixBio to elevate your laboratory capabilities with our innovative medical lab equipment, empowering you to drive progress in healthcare delivery.
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