Rev-CEM-Luc HIV Infection Reporter Cell Line

Rev-CEM-Luc HIV Infection Reporter Cell Line Description: Rev-CEM HIV Infection GFP/luciferase Reporter is Luciferase reporter cell line derived from CEM-SS. CEM-SS cells were infected with a Lentivirus pNL-GFP-RRE(SA). Clones were isolated and selected for HIV-dependent Luciferase expression.Rev-CEM expresses CD4, CXCR4, and low levels of CCR5. Infection by HIV results in Luciferase expression in 3-5 days. The cell line has been used to perform TCID50 assays on both X4 and R5 viruses. For routine measurements of infectivity with the Rev-CEM cells, we perform this assay in 96 well plates: 2 x 105 cells are infected with serially diluted virus in flat-bottom 96 well plates (final volume 200 ul RPMI + 10% FCS) and incubated for 3 to 5 days with fresh medium added as needed. The presence of GFP-positive cells is identified by fluorescent microscopy. HIV replication can also be followed by flow cytometry. Progenitor: Human T4-lymphoblastoid cell line, CEM-SS Phenotype: Expresses CD4, CXCR4 Applications: TCID50 assays, routine measurements of viral infectivity, anti-HIV drug screening, anti-HIV neutralizing antibody screening and quantification, HIV cell-cell transmission, HIV+ cell-derived exsomes Freezing Media: 20% FBS/RPMI with 10% DMSO. Propagation Media and Growth Condition: RPMI-1640 containing 10% FBS Split cells 1:2 every 1-2 days or 1:10 -1:20 every 2-3 days. Morphology: Round and clusters Detection: Firefly Luciferase System from Promega. Follow the manufacturers protocol for developing the assay (LUC). Contents & Storage: One vial of 6 x 10 ⁶ cells. Store it in liquid nitrogen freezer; IMPORTANT Cells are shipped frozen. If cells are not frozen upon arrival, contact VitaScientifc immediately. IMPORTANT Place frozen cells in liquid nitrogen until you are ready to thaw and propagate them. Warranty: Virongy warrants that cells shall be viable upon shipment from Virongy for a period of thirty days, provided they have been properly stored and handled during this period. Safety: BLS 2, Contains HIV Viral Elements Appropriate safety procedures should always be used with this material. Quality: Negative for mycoplasma, bacteria and fungi. Disclaimer: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reference: Wu, Y., Beddall, M. H., and Marsh, J. W. (2007). Rev-dependent indicator T cell line. Current HIV Research 5, 395-403 VirReport Cells Selection Guide HIV Infection Reporter cells Product Catalog No. Parental Cell Phenotype Reporter gene Rev-CEM-GFP HIV Infection Reporter Cell VRGC30001 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 GFP Rev-CEM-GFP/Luc HIV Infection Reporter Cell VRGC30002 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 Luciferase, GFP Rev-CEM-Luc HIV Infection Reporter Cell VRGC30003 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 Luciferase Rev-A3R5-GFP HIV Infection Reporter Cell VRGC30005 Human T4-Lymphoblastoid cell line, A3.01 CD4, CXCR4, 47 and CCR5 GFP Rev-A3-GFP/Luc HIV Infection Reporter Cell VRGC30009 Human T4-Lymphoblastoid cell line, A3.01 CD4, CXCR4 and 47 Luciferase, GFP