Rev-A3-GFP/Luc HIV Infection Reporter Cell Line

Rev-A3-GFP/Luc HIV Infection Reporter Cell Line Description: Infection of Rev-A3-GFP/LUC by HIV results in GFP or luciferase expression in 2-5 days. Rev-A3R5-GFP/LUC is permissive to subtype X4 utilizing primary isolates and infectious molecular clones. Rev-A3R5-GFP/LUC has no background GFP or luciferase expression in the absence of HIV infection, and is resistant to non-viral stimulations by mitogens, cytokines, or other cellular activators. Progenitor: Human T4-lymphoblastoid cell line, A3.01 Phenotype: Expresses CD4, CXCR4 and 47 Applications: TCID50 assays, routine measurements of viral infectivity, anti-HIV drug screening, anti-HIV neutralizing antibody screening and quantification, HIV cell-cell transmission, HIV+ cell-derived exsomes Freezing Media: 20% FBS/RPMI with 10% DMSO. Propagation Media and Growth Condition: RPMI-1640 containing 10-15% FBS, 1% L-Glut., 1% Pen./Strep., and 1mg/mL Geneticin (G418) Split 1:5, twice per week. Cells ready for infection after 2nd passage. Morphology: Round Detection: Flourescent microscopy or flow cytometetry (GFP). Firefly Luciferase System from Promega. Follow the manufacturers protocol for developing the assay (LUC). Contents & Storage: One vial of 6 x 10 ⁶ cells. Store it in liquid nitrogen freezer; IMPORTANT Cells are shipped frozen. If cells are not frozen upon arrival, contact VitaScientifc immediately. IMPORTANT Place frozen cells in liquid nitrogen until you are ready to thaw and propagate them. Warranty: Virongy warrants that cells shall be viable upon shipment from Virongy for a period of thirty days, provided they have been properly stored and handled during this period. Safety: BLS 2, Contains HIV Viral Elements Appropriate safety procedures should always be used with this material. Quality: Negative for mycoplasma, bacteria and fungi. Disclaimer: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reference: Montefiori DC, et al Magnitude and breadth of the neutralizing antibody response in the RV144 and Vax003 HIV-1 vaccine efficacy trials. J Infect Dis. 2012 Aug 1;206(3):431-41. VirReport Cells Selection Guide HIV Infection Reporter cells Product Catalog No. Parental Cell Phenotype Reporter gene Rev-CEM-GFP HIV Infection Reporter Cell VRGC30001 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 GFP Rev-CEM-GFP/Luc HIV Infection Reporter Cell VRGC30002 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 Luciferase, GFP Rev-CEM-Luc HIV Infection Reporter Cell VRGC30003 Human T4-Lymphoblastoid cell line, CEM-SS CD4 and CXCR4 Luciferase Rev-A3R5-GFP HIV Infection Reporter Cell VRGC30005 Human T4-Lymphoblastoid cell line, A3.01 CD4, CXCR4, 47 and CCR5 GFP Rev-A3-GFP/Luc HIV Infection Reporter Cell VRGC30009 Human T4-Lymphoblastoid cell line, A3.01 CD4, CXCR4 and 47 Luciferase, GFP