Ras-GRF1 (Phospho-Ser916) Rabbit Polyclonal Antibody, 100 ug

Background: Promotes the exchange of Ras-bound GDP by GTP. Product datasheet: Overview Product Description Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity. Tested Applications IHC Species Reactivities Human Mouse Immunogen Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1. Properties Storage Instructions Store at -20C Clonality Polyclonal Antibody References: RasGRF1 regulates the hypothalamic-pituitary-adrenal axis specifically in early-adolescent female mice. Uzturk BG, et al. J Endocrinol, 2015 Oct. PMID 26246084 Derangement of Ras-guanine nucleotide-releasing factor 1 (Ras-GRF1) and extracellular signal-regulated kinase (ERK) dependent striatal plasticity in L-DOPA-induced dyskinesia. Cerovic M, et al. Biol Psychiatry, 2015 Jan 15. PMID 24844602 Age-dependent role for Ras-GRF1 in the late stages of adult neurogenesis in the dentate gyrus. Darcy MJ, et al. Hippocampus, 2014 Mar. PMID 24174283 Free PMC Article Mice Lacking Ras-GRF1 Show Contextual Fear Conditioning but not Spatial Memory Impairments: Convergent Evidence from Two Independently Generated Mouse Mutant Lines. d'Isa R, et al. Front Behav Neurosci, 2011. PMID 22164138 Free PMC Article Novel RasGRF1-derived Tat-fused peptides inhibiting Ras-dependent proliferation and migration in mouse and human cancer cells. Sacco E, et al. Biotechnol Adv, 2012 Jan-Feb. PMID 21620943 Isolation of multiple mouse cDNAs with coding homology to Saccharomyces cerevisiae CDC25: identification of a region related to Bcr, Vav, Dbl and CDC24. Cen H., Lowy D.D. EMBO J. 11:4007-4015(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/cJ. Cloning by functional complementation of a mouse cDNA encoding a homologue of CDC25, a Saccharomyces cerevisiae RAS activator. Martegani E., Vanoni M., Zippel R., Coccetti P., Brambilla R., Ferrari C., Sturani E.P., Alberghina L. EMBO J. 11:2151-2157(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 791-1262. Strain: SWR/J. Tissue: Brain. Identification of a mammalian gene structurally and functionally related to the CDC25 gene of Saccharomyces cerevisiae. Wei W., Mosteller R.D., Sanyal P., Gonzales E., McKinney D., Dasgupta C., Li P., Liu B.-X., Broek D. Proc. Natl. Acad. Sci. U.S.A. 89:7100-7104(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1031-1226. Ras-specific exchange factor GRF: oligomerization through its Dbl homology domain and calcium-dependent activation of Raf. Anborgh P.H., Qian X., Papageorge A.G., Vass W.C., DeClue J.E., Lowy D.R. Mol. Cell. Biol. 19:4611-4622(1999) [PubMed] [Europe PMC] Cited for: FUNCTION, OLIGOMERIZATION, INTERACTION WITH RASGRF2, MUTAGENESIS OF LEU-263 AND 394-LEU--LEU-400. Cloning and characterization of mouse UBPy, a deubiquitinating enzyme that interacts with the ras guanine nucleotide exchange factor CDC25(Mm)/Ras-GRF1. Gnesutta N., Ceriani M., Innocenti M., Mauri I., Zippel R., Sturani E., Borgonovo B., Berruti G., Martegani E. J. Biol. Chem. 276:39448-39454(2001) [PubMed] [Europe PMC] Cited for: UBIQUITINATION, INTERACTION WITH USP8.