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Pre-stained Protein Ladder (10-180kDa), 250ul MEIP80501

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Pre-stained Protein Ladder (10-180kDa) 250ul Pre-stained Protein Marker: 10 bands 10 to 180 kDa

Storage: Upon receipt store at -20C. Product is shipped with an ice pack.

Storage Buffer: 62.5mM TrisH3PO4 (pH 7.5 at 25C), 1mM EDTA, 2% (w/v) SDS, 10mM DTT, 1mM NaN3 and 33% (v/v) glycerol.

【Product Description】 The Prestained Protein Ladder is a mixture of ten purified native proteins ranging from 10kDa to180kDa.The concentration of each protein is between0.2-0.4mg/ml. The 75KDa band is prestained red to facilitate identifying the position of each band. Two different chromophores are bound to the proteins, producing a brightly colored ladder (see website for product images). The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional reducing agent necessary.

Important Product Information: Do not boil the protein ladder.

The molecular weights of the proteins have a lot-to-lot variation of approximately 3%. In low-percentage gels (< 10%), the low-molecular weight proteins in the ladder may migrate with the dye front. The large proteins (> 100K) in the ladder may require longer transfer times or higher transfer voltages for Western blotting. The mobility of prestained proteins can vary in different SDS-PAGE buffer systems; however, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.

Procedure for Use in Polyacrylamide Gel Electrophoresis:

1. Thaw the ladder at room temperature. Do not boil protein ladder.

2. Mix gently and thoroughly to ensure that the solution is homogeneous.

3.Load an appropriate volume of the ladder onto the gel.

Mini-gel: 5uL per well (0.75-1.0mm thick) or 10uL per well (1.5mm thick)

Large gel: 10uL per well (0.75-1.0mm thick) or 20uL per well (1.5mm thick)

4. Return the unused protein ladder to -20C for up to one year or 4C for up to three months.

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Pre-stained Protein Ladder (10-180kDa), 250ul MEIP80501
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