PHENYLMETHYLSULFONYL FLUORIDE (PMSF), MOLECULAR BIOLOGY, 25G Description: PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins. PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25C than at 4C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature. Synonyms : Phenylmethylsulfonyl fluoride; PMSF; Phenylmethanesulfonyl fluoride; -Toluenesulfonyl fluoride MOLECULAR BIOLOGY GRADE Formula: C 7 H 7 FO 2 S MW: 174.19 g/mol Purity: 98.5% Melting Point: 91-94C White to slightly off white cyrstalline powder Storage/Handling: Store desiccated at 4C. Soluble in DMSO or 100% Ethanol. Protect from light. Hygroscopic.
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