NCI-H522 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) ,1.5ml

NCI-H522 Cell Avalanche Transfection Reagent (human lung carcinoma cell) The Transfection Reagent: NCI-H522 Cell Avalanche Transfection Reagent is a new, proprietary solution specifically designed for transfection on NCI-H522 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H522 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H522 Cell Avalanche Transfection Reagent for transfection on NCI-H522 cells. Features: Specifically optimized to deliver nucleic acids into NCI-H522 cells Highest efficiency to ensure experimental success Lowest Cellular Toxicity-maintain cell density and reduce experimental biases Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate Deliver single or multiple plasmids Free of animal-derived components Simplest and straightforward protocols to Increase experimental throughput Compatible with serum Active in serum-containing medium Suitable for Reverse Transfection Great pricing Data ： FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H522 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H522 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H522 Cell Avalanche Transfection Reagent. For Other Cells ： NCI-H522 Cell Avalanche Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. EKVX Cell HOP-92 Cell NCI-H226 Cell NCI-H322M Cell A549 Cell NCI-H23 Cell NCI-H1975 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. https://www.vitascientific.com/image/data/EZBT10301 NCI-H522 Cell Avalanche Transfection Reagent (human lung carcinoma cell)2.pdf