Max Sensitive ECL Western HRP Substrate, 100ml, MEIP80701

The Max Sensitive ECL Western HRP substrate is a highly sensitive and enhanced detection kit used in conjunction with horseradish peroxidase (HRP) in immunoblotting experiments. This product was developed based on a new generation of enhanced chemiluminescent substrates, which emit light upon the occurrence of a chemical reaction under the catalysis of HRP and can be used to detect biomacromolecules such as proteins immobilized on membranes. Its high sensitivity enables easy detection of antigens at the PG level (and even the FG level), strong persistence of the luminescent signal, and detection with either X-ray film exposure or a chemiluminescent imager.

Product components:

Max Sensitive ECL-A (Luminol Enhancer): 50ml

Max Sensitive ECL-B (Peroxide): 50ml

Notes:

1. Wear gloves and use clean equipment such as clean forceps during contact with the membrane to avoid protein contamination and high background.

2. Protected from light; the formulated substrate working solution for chemiluminescence detection can be stored stably at room temperature for 8 h. Sunlight or other strong light can affect the working fluid, so strong light should be avoided for a long time. Short exposures to normal laboratory lights do not affect the use of working fluids.

Note: since this product is the most sensitive commercially available ECL luminescent solution, please experiment the best dilution ratio of primary and secondary antibody to avoid overexposure ！

Procedure:

1. Rinse the Western blot membrane well after incubating with HRP labeled antibody.

2. Mix the Max Sensitive ECL-A liquid and Max Sensitive ECL-B liquid in an equal volume ratio, i.e., to obtain ECL working solution (present application); About 3-5 ml ECL working solution is required per 5 cm x 8 cm of blotting membrane.

Note: the tips for aspirating fluid A and fluid B must be separate.

3. The liquid on the surface of the blotted membrane is blotted dry on absorbent paper and spread on a plastic film.

4. Drop the well-coordinated ECL working liquid uniformly on the surface of blotting membrane, after reaction for about 2 min, remove the ECL working liquid.

5. Clamp the blot film between two layers of plastic film, make a radiograph, or place inside a luminescent imager to photograph.

Comparison of effects

Fig.1

1. Exposure result diagram of ECL luminescent liquid of some famous brand M company

2. The Max Sensitive ECL Western HRP substrate (MEIP80701) exposure result

3. The exposure result diagram of ECL luminescent liquid from T company.

Fig.2

Exposure result chart of ECL luminogen from a famous brand of T company (Left Side)

Exposure result chart of the Max Sensitive ECL Western HRP substrate (MEIP80701) (Right Side)

Frequently asked questions and Answers