MAPK1/3 (Phospho-Tyr205/222) Rabbit Polyclonal Antibody, 100 ug

Background: This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]Product datasheet: Overview Product Description The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. Tested Applications WB Image Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody #11825.The lane on the right is treated with the antigen-specific peptide. Species Reactivities Human Mouse Rat Immunogen Peptide sequence around phosphorylation site of tyrosine 205/222(K-G-Y(p)-T-K) derived from Human MAPK1/3 . Properties Storage Instructions Store at -20°C Clonality Polyclonal Antibody References: ERK2-Pyruvate Kinase Axis Permits Phorbol 12-Myristate 13-Acetate-induced Megakaryocyte Differentiation in K562 Cells. Chaman N, et al. J Biol Chem, 2015 Sep 25. PMID 26269597 Histamine activates p38 MAP kinase and alters local lamellipodia dynamics, reducing endothelial barrier integrity and eliciting central movement of actin fibers. Adderley SP, et al. Am J Physiol Cell Physiol, 2015 Jul 1. PMID 25948734 Erk2 but not Erk1 regulates crosstalk between Met and EGFR in squamous cell carcinoma cell lines. Gusenbauer S, et al. Mol Cancer, 2015 Mar 4. PMID 25884419 Free PMC Article Reversal of corticosteroid insensitivity by p38 MAPK inhibition in peripheral blood mononuclear cells from COPD. Khorasani N, et al. Int J Chron Obstruct Pulmon Dis, 2015. PMID 25678784 Free PMC Article Endothelial progenitor cells proliferated via MEK-dependent p42 MAPK signaling pathway. Sandra F, et al. Mol Cell Biochem, 2015 Feb. PMID 25421411