I10013, CELENA® S Plan Apochromatic Lens, Plan Apochromat Fluor 1.25x (NA 0.04, WD 3.7)

Description

To ensure the highest quality cell images, all CELENA® S objective lenses were made in Japan,

the birthplace of world-class optics.

Plan Achromatic Objectives

Plan achromatic objectives are corrected for axial chromatic aberration in two wavelengths, spherical aberration in the color

green, and flat-field aberration. The CELENA® S TC PlanAchro Ph objectives are long working distance objectives that are suitable for routine

bright field, phase contrast, and fluorescence imaging with most cell culture vessels. Plan Fluorite Objectives

Plan fluorite objectives provides higher level of correction and produced from advanced glass formulations. The CELENA® S TC PlanFluor

objectives are long working distance fluorite objectives designed to use with standard culture vessels. These objectives are suitable

for sensitive fluorescence imaging and higher resolution bright field imaging.

Plan Apochromatic Objectives

Apochromatic objectives represent the most highly corrected objective lens available today. These objectives are corrected for

chromatic aberration in 3-5 colors and for spherical aberration in multiple colors too. The CELENA® S Plan Apochromat Fluor objectives are

top level objective lens that can be used for the most sensitive fluorescence imaging and highest resolution imaging applications.

Plan Achromatic Phase Contrast Objectives

Cat # Objective NA WD Coverglass thickness correction (mm) Bright field Phase contrast LWD Immersion

I10001 TC PlanAchro 4x Ph 0.13 17.3 – O O O I10002 TC PlanAchro 10x Ph 0.25 7.6 1.0 O O O I10003 TC PlanAchro 20x Ph 0.4 7.0 1.0 O O O I10004 TC PlanAchro 40x Ph 0.65 2.8 1.0 O O O Plan Fluorite Objective

Cat # Objective NA WD Coverglass thickness correction (mm) Bright field Phase contrast LWD Immersion

I10005 TC PlanFluor 4x 0.13 17.5 – O O I10006 TC PlanFluor 10x 0.3 7.5 1.0 O O I10007 TC PlanFluor 20x 0.4 7.5 1.0 O O I10008 TC PlanFluor 40x 0.6 2.9 1.0 O O Plan Apochromatic Objectives

Cat # Objective NA WD Coverglass thickness correction (mm) Bright field Phase contrast LWD Immersion

I10013 Plan Apochromat Fluor 1.25x 0.04 3.7 – O I10014 Plan Apochromat Fluor 4x 0.13 17.2 – O I10009 Plan Apochromat Fluor 10x 0.3 8.6 0.17 O I100010 Plan Apochromat Fluor 20x 0.65 0.7 0.17 O I100011 Plan Apochromat Fluor 40x 0.8 0.2 0.17 O I100012 Plan Apochromat Fluor 100xOil 1.25 0.19 0.17 O Oil

LWD = Long Working Distance; suitable for use with culture vessels with bottom thickness around 1 mm (dishes, well plates, slide glass, flasks, etc.)

Ordering Information VitaScientific No. Manufacturer No. Description

Celena S Imaging System Packages

LGBD50001 CS20002 Celena S Imaging System (Starter Package)

1) Mechanical stage included

2) 4 objectives 3) 3 filter cubes

LGBD50002 CS20001 Celena S Imaging System Main Body (Only)

1) Mechanical stage included

2) No objectives 3) No filter cubes

LGBD50110 Computer Media Pack: 24 Inch HD LED Display, Wireless mouse and 128 GB USB drive

Lens Selection (Selection Guide )

Plan Achromatic Lens Set (Included in the Starter Package LGBD50001)

LGBD50010 I10001 TC PlanAchro 4x Ph (NA 0.13, WD 17.3)

LGBD50011 I10002 TC PlanAchro 10x Ph (NA 0.25, WD 7.6)

LGBD50012 I10003 TC PlanAchro 20x Ph (NA 0.4, WD 7.0)

LGBD50013 I10004 TC PlanAchro 40x Ph (NA 0.65, WD 2.8)

Plan Fluorite Lens Set

LGBD50020 I10005 TC PlanFluor 4x (NA 0.13, WD 17.5)

LGBD50021 I10006 TC PlanFluor 10x (NA 0.3, WD 7.5)

LGBD50022 I10007 TC PlanFluor 20x (NA 0.4, WD 7.5)

LGBD50023 I10008 TC PlanFluor 40x (NA 0.6, WD 2.9)

Plan Apochromatic Lens Set

LGBD50030 I10009 Plan Apochromat Fluor 10x (coverslip-corrected, NA 0.3, WD 8.6)

LGBD50031 I10010 Plan Apochromat Fluor 20x (coverslip-corrected, NA 0.65, WD 0.7)

LGBD50032 I10011 Plan Apochromat Fluor 40x (coverslip-corrected, NA 0.8, WD 0.2)

LGBD50033 I10012 Plan Apochromat Fluor oil 100x (coverslip-corrected, NA 1.25, WD 0.19)

LED Filter Cubes (Selection Guide )

LGBD50050 I10101 DAPI (Ex375/28, Em460/50)

LGBD50051 I10102 EGFP (Ex470/30, Em530/50)

LGBD50052 I10103 RFP (Ex530/40, Em605/55)

LGBD50053 I10104 mCherry (Ex300/25, Em645/75)

LGBD50054 I10105 ECFP (Ex436/20, Em480/40)

LGBD50055 I10106 EYFP (Ex500/20, Em535/30)

LGBD50056 I10107 DSRed (Ex530/40, Em620/60)

LGBD50057 I10108 Cy5 (Ex620/60, Em700/75)

LGBD50030 I10109 Cy7 (Ex710/75, Em810/90)

LGBD50059 I10110 Cy3/TRITC longpass (Ex530/40, Em570lp)

LGBD50060 I10111 GFP long pass (Ex470/40, Em500lp)

LGBD50061 I10112 Cy5 long pass (Ex620/60, Em665lp)

LGBD50062 I10113 Custom filters

Vessel Holders and Accessories

LGBD50100 I10201 Universal holder

LGBD50101 I10202 25mm X 75 mm slide, two positions

LGBD50102 I10203 35mm petri dishes, four positions

LGBD50103 I10204 60mm petri dishes, two positions

LGBD50104 I10205 100 mm dish, one position

LGBD50105 I10206 25cm2 Nunc T-25 flasks, two positions

LGBD50106 I10207 75cm2 Nunc T-75 flask, one position

LGBD50107 I10208 25cm2 BD/Greiner T-25 flasks, two positions

LGBD50108 I10209 75cm2 BD/Greiner T-75 flask, one position

LGBD50109 I10210 Glass hemocytometer, one position

LGBD50109 I10301 Phase Alignment Telescope

Documents

Celena S Digital Imaging System Brochure Lens Selection Guide LED Filter Cube Selection Guide Resources

External Links:

Description of digital microscope in Wikipedia

An open source image analysis software, ImageJ, can be used for z-stak 3d reconstruction. ImageJ can be downloaded at this page.

Articles of 3D/4D image reconstruction using ImageJ:

3D / 4D image reconstruction using ImageJ / Fiji – Encite

3D Processing and Analysis with ImageJ