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FluoroTrans® W PVDF Transfer Membranes, 0.2 micron pore size, 7.3 x 8.5 cm, 15/Pkg

FluoroTrans® W PVDF Transfer Membranes, 0.2 micron pore size, 7.3 x 8.5 cm, 15/Pkg

Store / Applications / Bioreagents / Gels, Syringe Filters and Membranes / Gels
$96.00
In stock
Product Details

This package contains 15 PVDF W membranes in the 0.20 micron pore size,Typical Thickness127 m (5.0 mils), precut to 7.3 x 8.5 cm dimensions. Sensitive Protein Detection with Low Background and Very Low Burnthrough Naturally hydrophobic polyvinylidene fluorideisIdeal for a wide variety of protein-analysis applications, these membranes with 0.2m pore sizes feature naturally hydrophobic polyvinylidene fluoride filter media. The FluoroTrans media are white, microporous solid phase supports that bind proteins tenaciously via hydrophobic interactions. The high surface area allows for strong hydrophobic interaction with proteins and typically adsorbs 50% more protein than nylon or nitrocellulose. Filter membranes are resistant to a wide range of chemical solvents (i.e., acetone, DMSO, dimethyl formamide) and will not shrink during destaining with methanol. Resistance to trifluoroacetic acid and triethylamine allows direct insertion of FluoroTrans membrane materials into amino acid analyzers and gas phase protein sequencing equipment. The FluoroTrans W membraneis optimized for Western transfer applications but can also be used for Southern transfers. This membrane allows for sensitive protein detection with low background and very low protein burn-through. Immobilized proteins can be visually detected with Coomassie blue, Amido Black, Ponceau S, and colloidal gold. The FluoroTrans PVDF membrane is optimized for N-terminal protein sequencing and features good signal-to-noise with standard detection systems. Immobilized proteins can be used directly for sequencing or visually detected with common staining reagents, including Amido Black, Ponceau S, and colloidal gold. Performance FluoroTrans Membrane Has Excellent Sensitivity, Signal,Background and Very Low Burnthrough in Western Transfers Rabbit reticulocyte lysate (Amersham) was loaded in lanes of polyacrylamide gels at f.s., 1/3 and 1/10 dilutions. After electrophoresis, proteins were transferred to membranes. Membranes were stained with 0.1% Amido Black, 45% methanol, and 2% acetic acid for 4 minutes; then destained for 5 minutes with two changes of 90% methanol and 2% acetic acid. Stained membranes were rinsed in water and air dried.

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FluoroTrans® W PVDF Transfer Membranes, 0.2 micron pore size, 7.3 x 8.5 cm, 15/Pkg
Store / Applications / Bioreagents / Gels, Syringe Filters and Membranes / Gels
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