ERK8 (Phospho-Thr175/Tyr177) Rabbit Polyclonal Antibody, 100 ug

Background: In vitro, phosphorylates MBP.Product datasheet: Overview Product Description In vitro, phosphorylates MBP. Tested Applications WB Image Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody #12105. The lane on the right is treated with the synthesized peptide. Species Reactivities Human Mouse Immunogen Peptide sequence around phosphorylation site of threonine 175 and tyrosine 177 (A-V-T(p)-E-Y(p)-V-A) derived from Human ERK8. Properties Storage Instructions Store at -20°C Clonality Polyclonal Antibody References: Structure prediction and validation of the ERK8 kinase domain. Strambi A, et al. PLoS One, 2013. PMID 23326322 Free PMC Article Regulation of the activity and expression of ERK8 by DNA damage. Klevernic IV, et al. FEBS Lett, 2009 Feb 18. PMID 19166846 Characterization of the reversible phosphorylation and activation of ERK8. Klevernic IV, et al. Biochem J, 2006 Feb 15. PMID 16336213 Free PMC Article A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA. Groehler AL, et al. J Cell Biol, 2010 Aug 23. PMID 20733054 Free PMC Article ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5. Saelzler MP, et al. J Biol Chem, 2006 Jun 16. PMID 16624805 ERK8, a new member of the mitogen-activated protein kinase family. Abe M.K., Saelzler M.P., Espinosa R. III, Kahle K.T., Hershenson M.B., Le Beau M.M., Rosner M.R.J. Biol. Chem. 277:16733-16743(2002) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH CSK, MUTAGENESIS OF LYS-42; THR-175 AND TYR-177, PHOSPHORYLATION AT THR-175 AND TYR-177, CHARACTERIZATION, FUNCTION. Tissue: Testis. Activation of the Erk8 mitogen-activated protein (MAP) kinase by RET/PTC3, a constitutively active form of the RET proto-oncogene. Iavarone C., Acunzo M., Carlomagno F., Catania A., Melillo R.M., Carlomagno S.M., Santoro M., Chiariello M.J. Biol. Chem. 281:10567-10576(2006) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION, INTERACTION WITH ABL1 AND RET. Tissue: Medulla oblongata. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5. Saelzler M.P., Spackman C.C., Liu Y., Martinez L.C., Harris J.P., Abe M.K.J. Biol. Chem. 281:16821-16832(2006) [PubMed] [Europe PMC] Cited for: INTERACTION WITH TGFB1I1. Large-scale proteomics analysis of the human kinome. Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].