Anti-c-myc Epitope Tag (EQKLISEEDL) Mouse Monoclonal Antibody (DBOA10004), 100 ug Background: A myc tagEQKLISEEDL is a peptide tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway. Product datasheet: Overview Product Name Anti-c‐myc Epitope Tag Mouse Monoclonal Antibody (DBOA10004), 100 ug Description Mouse Monoclonal Antibody (DBOA10004), against c‐myc Epitope Tag, can be used in identify myc tagged fusion protein in Western blotting, immunoprecipitation immunofluorescence microscopy or other immunoassays. Tested Applications WB, IHC Species Reactivities Reacts with: c-myc tag Immunogen c-myc tag peptideEQKLISEEDL Positive Control N/A Properties Form Freeze dried solid Storage Instructions Store at +4C long term. After reconstitution, Store at +4C short term (1-2 weeks). Aliquot and store at -20C long term. Avoid repeated freeze / thaw cycles. Storage Buffer When reconstituted with 100 deionized water, PBS with no preservatives. Constituent PBS Purity Purified by protein A Clonality Monoclonal Clone Name M1Y Isotype IgG1 Applications The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user. WB 1/500 - 1/2000. IHC 1/200 - 1/1000
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