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Anti PIK3CA(H1047Y) Mouse Monoclonal Antibody, 100 ul (26216)

Anti PIK3CA(H1047Y) Mouse Monoclonal Antibody, 100 ul (26216)

Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
$495.00
In stock
Product Details

PIK3CA(H1047Y) [26216] [100ul] Catalog Number: 26216 Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha Description: Anti PIK3CA(H1047Y) Mouse Monoclonal Antibody Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of H1047Y, human origin. Applications: ELISA, WB, IHC Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 Concentration: 0.5 mg/ml Host: Mouse Format: Liquid Clonality: Monoclonal Isotype: Purity: Purified from ascites Storage buffer: Preservative:no Constituents:PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes H1047Y mutant, but not wild-type PIK3CA of vertebrates. Storage Conditions: Store at -20C. Avoid freeze / thaw cycles Download Datasheet: Western blot: Western blot analysis of recombinant PIK3CA(H1047Y) and wildtype proteins. Purified His-tagged PIK3CA(H1047Y) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(H1047Y) monoclonal antibody (Cat. #26216). Immunofluorescence: Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(H1047Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(H1047Y) plasmid (right column), then fixed and stained with Anti PIK3CA(H1047Y) monoclonal antibody (Cat. #26216).

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Anti PIK3CA(H1047Y) Mouse Monoclonal Antibody, 100 ul (26216)
Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
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