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Anti IDH2(R172S) Mouse Monoclonal Antibody, 100 ul (26408)

Anti IDH2(R172S) Mouse Monoclonal Antibody, 100 ul (26408)

Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
$495.00
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Product Details

IDH2(R172S) [26408] [100ul] Catalog Number: 26408 Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH Description: Anti IDH2(R172S) Mouse Monoclonal Antibody Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172S IDH2 mutations do exist in diffusely infiltrative gliomas. Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172S, human origin. Applications: ELISA, WB, IHC Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 Concentration: 1 mg/ml Host: Mouse Format: Liquid Clonality: Monoclonal Isotype: Purity: Purified from ascites Storage buffer: Preservative:no Constituents:PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: recognizes R172S mutant, but not wild-type IDH2 of vertebrates. Storage Conditions: Store at -20C. Avoid freeze / thaw cycles Download Datasheet: Western blot: Western blot analysis of recombinant IDH2(R172S) and wildtype proteins. Purified His-tagged IDH2(R172S) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with Anti IDH2(R172S) monoclonal antibody (Cat. #26408). Immunofluorescence: Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172S) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2(R172S) plasmid, pCDNA3-GFP-IDH2 (WT) plasmid, pCDNA3-GFP-IDH2 (R172G) plasmid, pCDNA3-GFP-IDH2 (R172K) plasmid, pCDNA3-GFP-IDH2 (R172W) plasmid or pCDNA3-GFP-IDH2 (R172H) plasmid, then fixed and stained with Anti IDH2(R172S) monoclonal antibody (Cat. #26408)

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Anti IDH2(R172S) Mouse Monoclonal Antibody, 100 ul (26408)
Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
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