Anti-FGF2 Mouse Monoclonal Antibody [F2G] (DBOA10001), 100 ug

This is a mouse monoclonal IgM antibody against humna Fibroblast Growth Factor-2. Background FGF2,also known as Basic fibroblast growth factor, bFGF, or FGF- is a member of the fibroblast growth factor family [1]. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples. In this study, bFGF was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase.[3] Reference Kim HS (1998). "Assignment of the human basic fibroblast growth factor gene FGF2 to chromosome 4 band q26 by radiation hybrid mapping". Cytogenet. Cell Genet. 83 (1-2): 73. doi:10.1159/000015129. PMID 9925931. Khn MC, Willenberg HS, Schott M, Papewalis C, Stumpf U, Floh S, Scherbaum WA, Schinner S. (2012). "Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation.". Mol Cell Endocrinol. 349 (2): 180188. doi:10.1016/j.mce.2011.10.018. PMID 22040599. Product datasheet: Overview Product Name Anti-FGF2 Mouse Monoclonal Antibody (DBOA10001), 100 ug Description Mouse Monoclonal Antibody against human Fibroblast Growth Factor 2 (FGF2), (Clone F2G), 100 ug Tested Applications WB, IHC Species Reactivities Reacts with: Human FGF2 Immunogen Purified recombinant fragment of FGF2 expressed in E. Coli Positive Control N/A Properties Form Freeze dried solid Storage Instructions Store at +4C long term. After reconstitution, Store at +4C short term (1-2 weeks). Aliquot and store at -20C long term. Avoid repeated freeze / thaw cycles. Storage Buffer When reconstituted with 100 deionized water, PBS with no preservatives. Constituent PBS Purity Purified by precipitation Clonality Monoclonal Clone Number F2G Isotype IgM Applications The application notes include recommended starting dilutions; optimal dilutions or concentrations should be determined by the end user. WB 1/500 - 1/2000. Predicted molecular weight: 31 kDa IHC 1/200 - 1/1000