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Anti FBXW7(R479L) Mouse Monoclonal Antibody, 100 ul (26347)

Anti FBXW7(R479L) Mouse Monoclonal Antibody, 100 ul (26347)

Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
$495.00
In stock
Product Details

FBXW7(R479L) [26347] [100ul] Catalog Number: 26347 Gene Symbol: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 Description: Anti FBXW7(R479L) Mouse Monoclonal Antibody Background: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Immunogen: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin. Applications: ELISA, WB Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 Concentration: 1 mg/ml Host: Mouse Format: Liquid Clonality: Monoclonal Isotype: Purity: Purified from ascites Storage buffer: Preservative:no Constituents:PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes R479L mutant, but not wild-type FBXW7 of vertebrates. Storage Conditions: Store at -20C. Avoid freeze / thaw cycles Download Datasheet: Western blot: Western blot analysis of recombinant FBXW7(R479L) and wildtype proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347). Immunofluorescence: Immunofluorescence of cells expressing FBXW7 proteins with Anti FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti FBXW7(R479L) monoclonal antibody (Cat. #26347).

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Anti FBXW7(R479L) Mouse Monoclonal Antibody, 100 ul (26347)
Store / Antibodies and Immunodetection Reagents / Primary Antibodies / Antibodies for Biomarkers
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