EGFR (S768I) 26483 [100ul] Catalog Number: 26483 Gene Symbol: EGFR/HER1/ERBB1 Description: AntiEGFR(S768I)Mouse Monoclonal Antibody Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of S768I, human origin. Applications: ELISA, WB, IF, IHC Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:200 Concentration: 1 mg/ml Host: Mouse Format: Liquid Clonality: Monoclonal Isotype: Purity: Purified from ascites Storage buffer: Preservative:no Constituents:PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Recognizes EGFR (S768I), but not wild type EGFR from vertebrates. Storage Conditions: Store at -20C. Avoid freeze / thaw cycles Download Datasheet: Western blot: Western blot analysis of recombinant EGFR (S768I) and wildtype proteins. Purified His-tagged EGFR (S768I) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(S768I) mouse monoclonal antibody (Cat. #26483). Immunofluorescence: Immunofluorescence of cells expressing EGFR proteins with anti EGFR(S768I) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(S768I) plasmid (right column), then fixed and stained with anti- EGFR (S768I) monoclonal antibody (Cat. #26483).
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