Background ARF1 (ADP-ribosylation factor 1) is a member of the ARF superfamily. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Tested Applications ELISA, WB, IHC Source Species Rabbit Species Reactivity Recognizes Arf1, Arf2 and Arf3 of vertebrates. Datasheet Western Blot Western blot analysis of recombinant Arf1 protein. Purified His-tagged Arf1 protein was blotted with anti-Arf1 rabbit polyclonal antibody (Cat. #NEBA10096). Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-Arf1 polyclonal antibody (Cat. #NEBA10096). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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