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Anti-AKT1 Rabbit Polyclonal Antibody, 100 µl (21274)

$219.00
In stock
Product Details

Background AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. Tested Applications ELISA, WB, IHC Source Species Rabbit Species Reactivity Recognizes AKT1 of vertebrates. Datasheet Western Blot Western blot analysis of recombinant AKT1 protein. Purified His-tagged AKT1 protein was blotted with anti-AKT1 rabbit polyclonal antibody (Cat. #2127. Immunohistochemistry Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-AKT1 polyclonal antibody (Cat. #NEBA10089). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

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Anti-AKT1 Rabbit Polyclonal Antibody, 100 µl (21274)
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