Background AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. Tested Applications ELISA, WB, IHC Source Species Mouse Species Reactivity Recognizes E49K mutant, but not wild-type AKT1 of vertebrates. Datasheet Western Blot Western blot analysis of recombinant AKT1 (E49K) and wildtype proteins. Purified His-tagged AKT1 (E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti-AKT1 (E49K) monoclonal antibody (Cat. #NEBA10170). Immunofluorescence Immunofluorescence of cells expressing AKT1 proteins with anti-AKT1 (E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1 (E49K) plasmid (right column), then fixed and stained with anti-AKT1 (E49K) monoclonal antibody (Cat. #NEBA10170).
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